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Antibodies sure to the blocking peptide no more bind for the epitope within the target protein. By comparing the staining through the blocked antibody vs . the antibody by yourself, one can see which staining is certain.
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This information outlines more possible optimization of cellular and immunoassay parameters to ensure the best possible outcomes are...
This tutorial outlines more doable optimization of cellular and immunoassay parameters to ensure the very best results are...
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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Blocking peptides are peptides that bind specifically to the concentrate on antibody and block antibody binding. These peptide ordinarily incorporates the epitope identified by the antibody.
Target Information Cyclin E belongs to the really conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic party. Cyclin E varieties a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S stage boundary and is particularly degraded as cells progress through S section.
Overexpression of this gene has been noticed in many tumors, which ends up in chromosome instability, and thus may possibly contribute to tumorigenesis. This protein was observed to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a crucial purpose in selling cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been described. Two more splice variants were documented but in-depth nucleotide sequence details will not be yet obtainable.
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